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Dual Fluorescent Protein Excitation Flashlight

作者:美国nightsea 时间:2020-02-14 08:56:06浏览816 次

信息摘要:

只需一个简单的手电筒即可在您的转基因实验中筛选绿色和红色荧光蛋白。The NightSea DFP-1 双荧光蛋白手电筒包括两个 3W 高强度发光二极管,每个都配有定制的干涉滤光片。将开关单向转动,您有蓝光来激发 GFP,将它转向另一个方向,强烈的绿光激发 DsRed。DFP-1 带有两对阻隔滤光玻璃——一个与每个 LED 一起使用——可阻挡反射光并传输荧光。

NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight

 The Model DFP Dual Fluorescent Protein Flashlights include two different, switch-selectable high intensity LEDs for excitation of fluorescent proteins. For years the most popular fluorescent colors have been green (GFP, eGFP, fluorescein, etc.) and red (DsRed, TdTomato, etc.), and we offered only one version of the DFP, with a combination of blue and green LEDs for exciting these fluorophores. This is still our ‘standard’ DFP product, but we are now offering build-to-order combinations using any two of the excitation colors that are available with our popular Model SFA Stereo Microscope Fluorescence Adapter.

NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight

With every DFP light you will receive two pairs of barrier filter glasses and a convenient storage case. The glasses are well matched to the excitation so that they block the reflected excitation light while transmitting the fluorescence with high efficiency, providing excellent viewing contrast.
This is an convenient alternative to built in fluorescence for your microscopy and portable live fluorescent viewing needs.

NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight

Specifications:

Dimensions: 5 x 3 x 2
 Battery Type: 4 C-cell
 Bulb Type: Two high intensity 3W LED
 Burn Time: 4 hours at full power
 Lamp Life: 10,000 hours
 Matching filter glasses and carrying case Included

 NIGHTSEA DFP Dual Fluorescent Protein Excitation Flashlight

Filter Set
ExcitationEmissionFluorophores

RB – Royal Blue440-460nm500nm LPGFP, fluorescein, lucifer yellow, …

CY – Cyan490-515nm550nm LPYFP, Venus, …

GR – Green510-540nm600nm LPDsRed, TdTomato, RFP, …

VI – Violet400-415nm460nm LPBFP, CFP, …

UV – Ultraviolet360-380nm415nm LPDAPI, …

 

http://www.luyorgroup.com/Fluorescent-Protein-light-sources/LUYOR-3280-Fluorescence-Flashlight.html 

http://www.luyorgroup.com/Fluorescent-Protein-light-sources/LUYOR-3415RG.html 

DFP-1荧光手电筒只需一个简单的手电筒即可在您的转基因实验中筛选绿色和红色荧光蛋白。The NightSea DFP-1 双荧光蛋白手电筒包括两个 3W 高强度发光二极管,每个都配有定制的干涉滤光片。将开关单向转动,您有蓝光来激发 GFP,将它转向另一个方向,强烈的绿光激发 DsRed。DFP-1 带有两对阻隔滤光玻璃——一个与每个 LED 一起使用——可阻挡反射光并传输荧光。

DFP-1荧光手电筒

规格

电池:4 C 电池

灯泡类型:两个高强度3W LED

燃烧时间:>4小时全功率

LED灯寿命:>10,000小时

尺寸:5 英寸(317.5 像素)长 x 3 英寸(7.6 厘米)直径(不包括手枪式握把)

DFP-1荧光手电筒。

DFP-1 双荧光蛋白手电筒,美国Nightsea生产

DFP-1 Dual Fluorescent Protein Flashlight

DFP-1 双荧光蛋白手电筒的应用:

用于检测、筛选转绿色荧光蛋白(GFP)和红色荧光蛋白(DsRed)的植物、动物及微生物,如水稻、玉米、斑马鱼、小鼠、细菌、真菌等等。。。。

DFP-1 双荧光蛋白手电筒包含:双荧光手电筒、黄色滤光眼镜、红色滤光眼镜三个组件

DFP-1 双荧光蛋白手电筒组件的使用:

1.双荧光手电筒(激发光源):手电筒状激发光源可激发两种光源:

1)3W LED Royal Blue——400-460nm,蓝色光源,照射含绿色荧光蛋白的样本;

2)3W LED Green——510nm-540nm,绿色光源,照射含红色荧光蛋白的样本。

                       

2.滤光眼镜(眼镜两个):

选择蓝色光源激发光或绿色光源激发光照射样本后,

1)黄色滤光镜观测,滤光镜挡住反射光,只允许绿色荧光通过,从而可以观测到绿色荧光蛋白; 

2)红色滤光镜观测,滤光镜挡住反射光,只允许红色荧光通过,从而可以观测到红色荧光蛋白

注:

DFP-1 双荧光蛋白手电筒的标配是检测绿色荧光蛋白(GFP)和红色荧光蛋白(DsRed)

现在,您可以选择以下任何两种荧光装置,组装成您专属的DFP-xy 双荧光蛋白手电筒啦。

荧光装置型号

citation激发光

Emission发射光

可观测荧光
RB-Royal Blue440-460nm500nm LPGFP,eGFP,fluorescein....
CY-Cyan490-515nm550nm LPYFP,Venus,Lucifer Yellow...
GR-Green510-540nm600nm LPDsRed,dTomato...
VI-Violet400-415nm460nm LPCFP,BFP...
UV-Ultra Violet360-380nm415nm LPDAPI

DFP-1双荧光蛋白手电筒的产品优势:

1.小巧、携带方便,便于野外检测

2.检测效率高,黑暗中检测一目了然

3.LED寿命10000h,且系统稳定,可用于长时间(>4h)使用

4.直接检测,而无需用底物显色,所以安全,对被检测对象无伤

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